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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be noticed in several tumors, which leads to chromosome instability, and thus may add to tumorigenesis. This protein was located to affiliate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important part in advertising mobile-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are described. Two additional splice variants were being noted but detailed nucleotide sequence facts just isn't nonetheless offered.
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Blocking peptides are peptides that bind exclusively into the target antibody and block antibody binding. These peptide normally contains the epitope acknowledged because of the antibody.
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This guideline outlines more attainable optimization of mobile and immunoassay parameters to make certain the absolute best outcomes are...
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This tutorial outlines further doable optimization of mobile and immunoassay parameters to make sure the very best results are...
Antibodies certain to the blocking peptide now not bind to the epitope within the focus on protein. By evaluating the staining within the blocked antibody versus the antibody alone, one can see which staining is distinct.
Focus on Information and facts Cyclin E belongs to the extremely conserved cyclin loved ones, whose associates are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins functionality as regulators of CDK kinases. Different cyclins show unique expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E kinds a complex with and capabilities to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and is also degraded as cells development by S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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